John married Mary Ruthven on 24 Jan 1579 in Perth, Perth, Scotland. Mary (daughter of William Ruthvern and Lady Dorothea Stewart, of Avondale) was born on 1 Jul 1562 in Ruthven Castle, Perthshire, Scotland; died on 19 Mar 1613 in Perth, Perthshire, Scotland. [Group Sheet] [Family Chart]

1. Baroness Anne Stewart was born in 1591 in Atholl, Perthshire, Scotland; died on 15 Oct 1635 in Perth, Perthshire, Scotland.
2. Lady Dorothea Stewart was born on 1 Jan 1581 in Atholl, Perthshire, Scotland; died on 6 Aug 1628 in Tullibardine, Perth - Perthshire, Scotland.
3. Jean Stewart was born in 1587 in Atholl, Perthshire, Scotland; died on 19 Jul 1623 in Scotland.
4. George Stewart was born about 1589 in ; died before 1595.
5. David Stewart was born about 1591 in .
6. Mary Stewart was born about 1595 in Midlothian, Scotland; died in 1639 in Midlothian, Scotland.
7. John Stewart was born about 1597 in Atholl, Perth, Scotland.

John married Lady Margaret Fleming, Duchess of Albany and Countess of Atholl in 1551 in Blair Castle, Blair Atholl, Perthshire, Scotland. Margaret was born in 1536 in Biggar, Lanarkshire, Scotland; died on 15 Mar 1586 in Kincardine Castle, Kincardine O'Neil, Perthshire, Scotland; was buried on 18 Mar 1586 in Edinburgh, Midlothian, Scotland. [Group Sheet] [Family Chart]