1535 - 1604 (69 years)
| John Hamilton, 4th Earl of Arran, 1st Marquess of Hamilton | |
| B: | 1535 |
| Arran, Bute, Butshire, Scotland | |
| D: | 6 Apr 1604 |
| Isle of Arran, Firth Clyde, Bute, Scotland | |
 | Margaret Lyon 1551-1626 |
| Margaret Lyon | |
| B: | 15 Jun 1551 |
| Glamis Castle, Angus, Scotland | |
| M: | 30 Dec 1577 |
| Hamilton, Lanark, Scotland, United Kingdom | |
| D: | 23 Nov 1626 |
| Avondale, Lanarkshire, Scotland | |
