| Children |
| | 1. Lady Janet Stewart, b. Traquair Castle, Scotland  d. Dawyck Castle, Peeblesshire, Scotland |
| | 2. Robert Stewart, b. 1552, Traquair, Peebles-shire, Scotland d. Feb 1623, Scotland, United Kingdom (Age 71 years) |
| | 3. William Stewart, b. Abt 1553, Traquair, Peeblesshire, Scotland d. 20 May 1605 (Age ~ 52 years) |
| | 4. Lady Mary elspeth Stewart, b. 1555, Perthshire, Scotland d. 28 Feb 1606, Scotland (Age 51 years) |
| | 5. Walter Stewart, 8th Baron of Kincardine, b. Abt 1555, Traquair, Peebles-shire, Scotland, United Kingdom d. 1640 (Age ~ 85 years) |
| | 6. Janet Stewart, b. 1 Nov 1555 d. 28 Feb 1606 (Age 50 years) |
| | 7. Mary Anne Stewart, b. From 1544 to 1570 d. 18 Feb 1587 (Age ~ 43 years) |
| | 8. James Stewart, b. Abt 1558, County Waterford, Ireland |
| | 9. Janet Stewart, b. 1 Nov 1558, Traquhair, Peeblesshire, Scotland d. 28 Feb 1606, Dawyck House, Peebles-shire, Scotland, United Kingdom (Age 47 years) |
| | 10. Isabel Stuart, b. Abt 1559, Traquair, Peebles-shire, Scotland, United Kingdom |
| | 11. Margaret Kelly Stewart, b. 1562, Traquair, Peeblesshire, Scotland d. 21 Dec 1625, Manor, Peeblesshire, Scotland (Age 63 years) |
| | 12. Mary Stewart, b. Abt 1564, Traquair, Peebles-shire, Scotland |
| | 13. Gelis Stewart, b. 1566, Traquair, Peeblesshire, Scotland d. 14 Nov 1624, Wrightslands, , , Scotland (Age 58 years) |
| | 14. #, b. 1576, Beith, Ayrshire, Scotland d. 30 Jul 1607, Loneheid Lochwinnoch Saint Ninians, Stirlingshire, Scotland (Age 31 years) |
| | 15. John Stewart, b. 1594, Scotland, United Kingdom |
| | 16. William Charles Stewart, b. 1605, Scotland d. 1682, Virginia, United States (Age 77 years) |
|
1538 - 1606 (68 years)
